訂貨：，，

EZ-Link（R） NHS-PEG4-Biotin,No-Weigh（tm） Format介紹

Thermo Scientific BCA蛋白定量產品可以用于研究蛋白：蛋白間相互作用，衡量親和層析后的組分含量，估算從細胞裂解液里提取的膜蛋白含量和高通量篩選融合蛋白。

EZ-Link（R） NHS-PEG4-Biotin,No-Weigh（tm） Format現貨供應

EZ-Link（R） NHS-PEG4-Biotin,No-Weigh（tm） Format品牌介紹

美國Pierce公司（www.pierce.com）是蛋白質化學、免疫學和層析等很多方面的和*。http://www.thermo.com.cn/Category828.html

主要產品包括：

1）化學發(fā)光體和比色底物

2）分析試劑

3）熒光標記探針

4）酶聯免疫分析試劑盒等

EZ-Link（R） NHS-PEG4-Biotin,No-Weigh（tm） Format相關產品

EZ-Link（R） NHS-PEG4-Biotin,No-Weigh（tm） Format

僅用于科研

Thermo Scientific EZ-Link NHS-PEG4-Biotin is a pegylated, water-soluble reagent for simple and efficient biotin labeling of antibodies, proteins and other primary amine-containing macromolecules.

The N-hydroxysuccinimide ester （NHS） group reacts specifically and efficiently with lysine and N-terminal amino groups at pH 7-9 to form stable amide bonds. The hydrophilic polyethylene glycol （PEG） spacer arm of this biotinylation reagent imparts water solubility that is transferred to the biotinylated molecule, thus reducing aggregation of labeled proteins stored in solution （read more about PEGylation）. The PEG spacer arm also gives the reagent a long and flexible connection to minimize steric hindrance involved with binding to avidin molecules.

Highlights:

• Label almost any protein with biotin to facilitate immobilization, purification or detection
• Amine-reactive NHS ester reacts rapidly with any primary amine-containing molecule to attach the biotin label via a stable amide bond
• Reagent is water soluble and membrane impermeable – suitable for cell surface labeling
• PEGylation increases solubility and helps prevent aggregation of labeled molecules
• Long spacer arm reduces steric hindrance when binding to avidin molecules
• No-Weigh Format saves time, preserves reagent activity and prevents cross-contamination between uses
• Reactive group: N-hydroxysuccinimide ester （NHS ester）

• Reactive toward: primary amines, as in side chain of lysine （K） and N-terminus of polypeptides