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Thermo Scientific DyLight 488 Amine-Reactive Dye is an NHS ester-activated derivative of high-performance DyLight 488 for fluorescent labeling of antibodies and other proteins to be used as molecular probes for cellular imaging and other fluorescence detection methods.

DyLight 488 has high fluorescence intensity over a broad pH range (pH 4-9) and is more photostable than Cy2, Alexa Fluor 488, FITC and LI-COR* Dyes in many applications. The high water solubility of DyLight Fluors allows a high dye-to-protein ratio to be achieved without causing precipitation of the conjugates. DyLight 488 Amine-Reactive Dye is available as a stand-alone reagent or as part of two antibody labeling kit sizes.

Highlights:

• High performance – DyLight 488 is comparable to Alexa Fluor 488 and brighter than FITC and Cy2
• Specific – NHS ester-activated dye labels proteins and other molecules at primary amines (-NH2)
• Convenient kit sizes – standard and microscale sizes are offered to match your experimental needs
• Optimized procedure – following the standard protocol results in antibodies with excellent dye:protein ratios and recovery rates for optimum activity and fluorescence labeling