PIERCE,N-ETHYLMALEIMIDE
- 型號： 23030
- 更新時間： 2024-05-31
- 上一個：23011PIERCE,（MMTS） Methyl methanethiolsulfonate, 200 mg
- 下一個：23208PIERCE,Pre-Diluted Protein Assay Standards:Bovin
訂貨：，，
N-ETHYLMALEIMIDE介紹
Thermo Scientific BCA蛋白定量產品可以用于研究蛋白：蛋白間相互作用，衡量親和層析后的組分含量，估算從細胞裂解液里提取的膜蛋白含量和高通量篩選融合蛋白。
N-ETHYLMALEIMIDE現貨供應
PIERCE | 23030 | N-ETHYLMALEIMIDE | 25 GM |
PIERCE | 23208 | Pre-Diluted Protein Assay Standards: Bovine Serum Albumin （ | KIT |
PIERCE | 23209 | ALBUMIN STANDARD, 10 X 1 ML | 10 X 1 ML |
N-ETHYLMALEIMIDE品牌介紹
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N-ETHYLMALEIMIDE僅用于科研
Thermo Scientific Pierce N-Ethylmaleimide （NEM） is a small compound that forms stable, covalent thioether bonds with sulfhydryls （e.g., reduced cysteines）, enabling them to be permanently blocked to prevent disulfide bond formation.
N-Ethylmaleimide （NEM） is an alkylating reagent that reacts with sulfhydryls. At pH 6.5-7.5, the maleimide reaction is specific for sulfhdryls; however, at pH values greater than 7.5, reactivity with amino groups occurs. Maleimide groups react with sulfhydryls by nucleophilic attack of the thiolate anion on one of the carbons of the double bond. When sufficient sulfhydryls have been blocked, the reaction can be monitored by measuring the decrease in absorbance at 300nm as the double bond becomes a single bond. The resulting thioether group is non-reversible and terminates in an ethyl group, blocking or capping the sulfhydryl.
Highlights:
- Permanently block sulfhydryls to prevent disulfide bond formation.
- Monitor the reaction by measuring the decrease in absorbance at 300nm
- Block sulfhydryl-containing reagents that interfere in enzyme assays
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