PIERCE，Ellman's Reagent, 5,5'-Dithio-bis-（2-nitrob
- 型號(hào)： 22582
- 更新時(shí)間： 2024-05-31
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Ellman's Reagent, 5,5'-Dithio-bis-（2-nitrobenzoic Acid）介紹
Thermo Scientific BCA蛋白定量產(chǎn)品可以用于研究蛋白：蛋白間相互作用，衡量親和層析后的組分含量，估算從細(xì)胞裂解液里提取的膜蛋白含量和高通量篩選融合蛋白。
Ellman's Reagent, 5,5'-Dithio-bis-（2-nitrobenzoic Acid）現(xiàn)貨供應(yīng)
PIERCE | 22582 | Ellman's Reagent, 5,5'-Dithio-bis-（2-nitrobenzoic Acid） | 5 GM |
PIERCE | 22585 | DITHIOBIS （SUCCINIMIDYL PROPIONATE） （DSP） 1 GM | 1 GM |
PIERCE | 22586 | DSP （Lomant's Reagent）, 50 mg | 50 MG |
Ellman's Reagent, 5,5'-Dithio-bis-（2-nitrobenzoic Acid）品牌介紹
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Thermo Scientific Pierce DTNB （Ellman's Reagent） reacts with sulfhydryl groups to yield a colored product, providing a reliable method to measure reduced cysteines and other free sulfhydryls in solution.
Ellman's Reagent （5,5'-dithio-bis-[2-nitrobenzoic acid]） is used to estimate sulfhydryl groups in a sample by comparing to a standard curve of a sulfhydryl-containing compound such as cysteine. Alternatively, sulfhydryl groups can be assayed using the extinction coefficient of TNB （14,150M-1cm-1 at 412nm）. Ellman's Reagent has been used for applications other than sulfhydryl estimation, such as for determining alkylthiols by HPLC using a pre-column derivatization procedure and to study thiols in the active site of several enzymes.
Highlights:
- Determine availability of sulfhydryls for labeling or crosslinking applications
- Assay by measuring the absorbance at 412nm or using cysteine as a standard
- Temporarily block sulfhydryls to perform chemistries on other protein functionalities
Ellman's Reagent, 5,5'-Dithio-bis-（2-nitrobenzoic Acid）僅用于科研
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