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SULFOLINK COUPLING GEL介紹

Thermo Scientific BCA蛋白定量產品可以用于研究蛋白：蛋白間相互作用，衡量親和層析后的組分含量，估算從細胞裂解液里提取的膜蛋白含量和高通量篩選融合蛋白。

SULFOLINK COUPLING GEL現貨供應

SULFOLINK COUPLING GEL品牌介紹

美國Pierce公司（www.pierce.com）是蛋白質化學、免疫學和層析等很多方面的和*。http://www.thermo.com.cn/Category828.html

主要產品包括：

1）化學發光體和比色底物

2）分析試劑

3）熒光標記探針

4）酶聯免疫分析試劑盒等

SULFOLINK COUPLING GEL僅用于科研

Thermo Scientific SulfoLink Coupling Resin is porous, crosslinked, 6% beaded agarose that has been activated with iodoacetyl groups for covalent immobilization of cysteine-peptides and other sulfhydryl molecules.

When incubated with a solution of peptide or protein that contains reduced cysteine residues, the iodoacetyl groups of SulfoLink Resin react specifically and efficiently with the exposed sulfhydryls （-SH） to form covalent and irreversible thioether bonds that permanently attach the peptide or protein to the resin. The result is a custom-made affinity resin for purification of antibodies, antigens and other molecules of interest. Choose one of two convenient SulfoLink Immobilization Kits for peptides or proteins. Each kit contains all the reagents for preparing the sample and five columns containing 2mL of SulfoLink Resin that can be used in either gravity-flow or centrifuge format for efficient coupling reactions and multiple cycles of affinity purification. A single-column Trial Kit for use with either peptides or proteins is also available, or the SulfoLink Coupling Resin can be purchased separay.

Highlights:

• Specific conjugation through sulfhydryl （-SH） groups – The iodoacetyl groups react specifically with sulhydryls to form irreversible thioether bonds
• Separate kits optimized for peptides or proteins – Kits include optimized reagents for preparing peptide or protein samples for efficient immobilization
• Fast – Spin columns increase protocol speed; prepare and couple samples in 2 hours （peptides） to 3.5 hours （proteins）
• Flexible coupling conditions – Use pH 7.5-9.0 aqueous buffers, organic solvent （e.g., 20% DMSO） or denaturant （guanidine•HCl）, as needed for protein or peptide solubility during coupling reaction
• Easy-to-follow instructions – streamlined protocols for sample preparation, immobilization, and affinity purification

  High capacity – Immobilize 1-2 mg peptide or 2-20 mg protein per 2-ml column of SulfoLink Coupling Resin