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PIERCE 15128Reacti-Bind (tm) NeutrAvidin (tm) Coated Polystyrene Strip

更新時間:2013-07-24      點(diǎn)擊:1523

產(chǎn)品名稱:Reacti-Bind (tm) NeutrAvidin (tm) Coated Polystyrene Strip
產(chǎn)品貨號:PIERCE 15128
產(chǎn)品規(guī)格:5 PLATES/EA
產(chǎn)品價格:1910
產(chǎn)品說明書地址:http://www.piercenet.com/

Pierce Streptavidin coated plates and Neutravidin coated plates.

Thermo Scientific Pierce Streptavidin and NeutrAvidin Coated Plates are pre-blocked and ready-to-use polystyrene 96-well and 384-well plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays.

Thermo Scientific Pierce Streptavidin and NeutrAvidin Coated Plates are available in 96-well and 384-well plate formats and pre-blocked with BSA or SuperBlock Blocking Buffers. These Pierce Biotin-Binding Plates are available in clear, white and black plates can be used with colorimetric, chemiluminescent and fluorescent detection methods, respectively.

Highlights:

  • Easy and gentle immobilization of biotin-containing conjugates
  • Low nonspecific binding
  • No denaturing of the protein component of a conjugate upon binding
  • Ideal for binding small biotinylated hydrophilic molecules (e.g., peptides) that typically exhibit poor binding to polystyrene
  • Pre-blocked with your choice of Blocker BSA or SuperBlock Blocking Buffer

Additional Highlights of NeutrAvidin Coated Plates:

  • Less expensive than streptavidin coated plates
  • Lowest nonspecific binding properties of all biotin-binding proteins

Product Details:

Plate Characteristics for Standard Capacity Biotin-Binding Plates.
Biotin-Binding
Protein
Plate Type Coat Volume** Block Volume** Approximate Binding Capacity
NeutrAvidin
96-Well,
8-Well Strip
100µL/well

200µL/well

~15pmol biotin/well
384-Well
50µL/well
100µL/well
~10pmol biotin/well
Streptavidin
96-Well,
8-Well Strip
100µL/well
200µL/well
~5pmol biotin/well
384-Well
50µL/well
100µL/well
~4pmol biotin/well
** The coating and blocking volumes give here are typical. Additional variations are also available.

Streptavidin and NeutrAvidin Protein are not glycosylated which prevents nonspecific lectin-binding observed with avidin coated plates. Biotin-binding plates are ideal for applications when direct adsorption to polystyrene denatures antibodies or the target molecule. Streptavidin has an isoelectric point of 5-6, resulting in low nonspecific interactions. NeutrAvidin Protein has a near-neutral pI (6.3), resulting in even lower nonspecific interactions. NeutrAvidin Protein has an additional advantage of lacking the bacterial RYD sequence found on streptavidin, which eliminates nonspecific binding due to the RGD binding domain of adhesion receptors present in a variety of cells. NeutrAvidin Protein has the lowest nonspecific binding among the known biotin-binding proteins.

Related Products and Links:

High Binding Capacity Biotin-Binding Plates
High Sensitivity Biotin-Binding Plates
SuperBlock Blocking Buffer – or browse all blocking buffers
Secondary Antibody Selection Guide
ELISA Substrate Selection Guide
ELISA Accessories – microplate sealing tape, reagent reservoirs, microplates

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865512910、2581846065
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